Tang Muhan from Guangdong topped the women's 400m freestyle in 4:07.04, and Ma Yonghui and Zhang Ke, both from Hebei, took second and third places respectively.
The round of games kicked off with Valencia producing a solid defensive display to take all three points from their visit to San Sebastian with a 1-0 win away to Real Sociedad.
Miguel Angel Russo's men are now four points clear at the top of the Group H standings, with one match remaining before the knockout rounds.
"We faced a team that attacked with many players and it was a different match compared to the one against Sampdoria. We wanted to keep the line tight, put pressure on the central players and exploit the one-on-one in attack, but we weren't always able to find solutions, after all we were playing against a solid team and we knew we would have difficulties," said Pirlo.
After having won the 200m and 400m freestyle, Ji Xinjie from Shandong scooped his third gold of the competition in the men's 800m freestyle, with teammate Hu Jia finishing second and Zhang Ziyang from Hubei taking the bronze medal.
Pliskova wasted eight set points in the opener but recovered quickly in the following two sets, serving out the contest on Court Philippe-Chatrier after two hours and 15 minutes.
The top four of each group will play the second phase between October 16-December 12 in Suzhou.
The swimmers' performances at the championships will act as an important reference for their qualification for next year's Tokyo Olympic Games. Enditem
Rookie Yang Junxuan also claimed her second gold in the women's 200m freestyle in one minute and 56.52 seconds, Hou Yujie from Henan ranked first in the men's 100m freestyle, and Hebei's Zhang Ke touched the finishing line first in the women's 800m freestyle.
AC Milan continued their good form as they won 2-0 away to Crotone, Franck Kessie's penalty put the Rossoneri in charge in the first half, while new arrival Brahim Diaz sealed matters after the break.
In the other game in the Chengdu division, after a goalless first half, Taizhou Yuanda broke the deadlock in the 54th minute to claim a 1-0 victory against Beijing Renhe, and Beijing BSU defeated Suzhou Dongwu 3-2.